Critical Review of On Coffee Talk and Break-Room Chatter: Perceptions of Women Who Gossip in the Workplace

Farley, Timme, and Hart's (2010) article describes a study, which investigated the perceptions of female gossipers within the workplace. Approximately 500 students were asked to complete an online survey, 87 of whom (aged from 23 to 64) completed all 54 items. A questionnaire was conducted which included demographic items, and the subscales of a modified FIRO-B. Participants were arbitrarily allocated to a condition and were asked to â€Å"think of a female co-worker who frequently or rarely contributes negative information about other people during conversation† (Farley et al. p. 365) and then evaluate the target using a modified version of Schutz’s (1958) FIRO-B, which is a measuring instrument that contains six scales of nine-item questions (cited in Farley et al, 2010). Participants then judged the female co-worker on a five-point Likert-Type scale. The results showed that high gossipers were rated as needing to express more control than low gossipers. The participa nts also rated the high gossipers as wanting others to control them less than low gossipers.Furthermore, high gossipers were rated as less emotionally close with their peers than low gossipers. These findings support the hypothesis that high gossipers would obtain higher ratings on the express control dimension than low gossipers. The results also support the hypothesis that high gossipers would be rated as less likely to want others to put forth power over them than low gossipers. Farley et al. ‘s (2010) experiment needs a small degree of critiquing. For one, it uses a poor assortment of participants, as the sample is too constricted to make any real generalizations.Participants were recruited via email. This method of assignment would have led to unequal groups in terms of demographic characteristics such as different ages and gender (cited in Farley et al, 2010). Older people may be more judgmental of gossiping than younger people; therefore this may hinder the final result s. Also, the experimenter only used females in the questionnaire, drawing on the myth that the majority of gossipers are females; therefore it does not generalize to everyone in the workplace.There may well have been an interaction effect between gossipers and gender; therefore males as well as females should have been included in the questionnaire. Furthermore, I am not satisfied with how the authors defined ‘gossip’; in fact there is no clear statement delineating ‘gossip’. The lack of the experimenters’ control over the independent variable (gender) makes it a subject variable as opposed to a manipulated variable, therefore it is a quasi experiment and you cannot infer causality from the results.

Kant on Milgram’s Perils of Obedience

Stanley Milgram conducted a study through a laboratory set-up to evaluate the perils of obedience of different subjects and participants of the study. One of significant results of his study entails that ordinary people, his participants, actively performs his or her job without any hostility and regard in their participation on something wrong done. (Milgram, 1974). To some evaluated participants, the case describes their disobedience with the standards of morality given the provision of a governing or instructing authority. Participants who were made to do wrong at some point, would derive satisfaction from the wrongness by knowing that they obeyed the authority commanding their actions. Milgram mentions that obedience can be defined as the ability of an individual to disassociate his responsibility on the wrong act because he only granted his authority’s wishes (Milgram, 1974). Moreover, a subordinate would feel shame or pride depending on how he has followed an authority’s instructions. The reality this reflects in the society maintains that loyalty, duty and discipline are significantly strained with the emotional and obedience conflicts. A subordinate’s defined role is does not necessarily instill his morale, but rather socially construct his role based on the social provisions, including defiance of his role in the perspective of his authority. Obedience in some cases also reflects a defense for people to do wrong things, as explained in the article, many participants who became the teacher in the set-up, punished the learner because they were following the instructions of the experimenter, and assured of no responsibility with the learner. People heeds to authority without discerning their own stance or the consequences of their actions. Based on Kant’s model of society, all disobedience from the supreme legislative power, or the authority and law, is considered as the greatest and most punishable crime of mankind because it inhibits the very foundations of society. Kant’s position on obedience does not allow the challenge of authority, and rejects the right of revolution or rebellion. (Williams, 1983) The evidence is shown through the study conducted by Milgram. Kant strictly attributed authority with the same governing laws and constitution of a society, thus disallowing any form of disobedience. Realized through Milgram’s article is the conflict which arises from following the authority with personal moral issues within an individual. Kant’s philosophy on this maintains the duty of all individual to hope for both moral and positive law to be achieved. (Williams, 1983) Kant defined obedience as a requirement of pure reason from individuals and makes them coerce with the law of an authority, while maintaining their freedom within and among their fellows. (Williams, 1983) The system of an authority demands strict adherence from the subjects of the state, because that makes individuals be the subject of authority. However, Kant subjects a restriction to an individual to resist conflict of the morality of one’s own, with his adherence to the authority. The arguments resolved by Milgram on his study entails how individuals refer to their morality with obedience to authority. Evidently, emotions and moral issues comes in conflict with being submissive to authority, but in the end, people act even in contrast with their emotion because the pride and satisfaction of doing what can be considered as wrong, comes from following the directives of the authority.

Supply Chain Management in the Healthcare Institution Research Paper - 5

Supply Chain Management in the Healthcare Institution - Research Paper Example This can be attributed to the fact that world health bodies frequently update the global stock in medical supplies depending on the emerging trends (Toba, Tomasini & Farhan, 2008). Hospitals rely on these changes in the ordering of medical supplies. However, the quality of the supplies does not create the main problem in supply chain management, but the activities applied to the supply chain management. The difference in the strategies used in supply chain management creates the difference in the cost and efficiency of the process (Toba, Tomasini & Farhan, 2008). Disregard of the process of supply chain management creates the main challenge in making the process efficient and quality. To overcome these challenges Toba, Tomasini & Farhan (2008) suggest that managers should update their technological position and emerging trends in supply chain management. This will create an organization with a lot of sensitivity in terms of coming up with effective logistics procedures. Additionally, this will open up opportunities for the organization to refer to supply chain management bodies and programs that will aid in transforming the state of supply management systems in organizations. Other significant challenges in supply chain management in healthcare institutions include outdated IT systems, lack of executive involvement, poor infrastructure, poor distribution and inventory management and non-improvement of the procurement process (Toba, Tomasini & Farhan, 2008). In overcoming challenges in decision-making processes regarding purchasing decisions, Toba, Tomasini & Farhan (2008) advise that institutions should disregard the majority rule. This mode of making decisions should be replaced by the consensus method which minimizes the occurrence of misunderstandings and opposition. This also increases the compliance rate from physicians.

The Role of an Oncology Nurse in USA Research Paper

The Role of an Oncology Nurse in USA - Research Paper Example 1.2 The Role of an Oncology Nurse in USA The role of oncology nurses differs in relation to intensive care which has more focus on bone marrow transplantation and on cancer detection, screening and prevention. Practice of oncology includes participating in nursing research studies, making educational curricula, serving the patients as consultants and performing functions of executive. The main emphasis in all these roles is to provide nursing care to patients, planning, evaluation and nursing diagnosis. This process helps nurses to take a systematic and organized approach in the treatment of cancer patients. The role of oncology nurses is related to professionalism rather than just an occupation for instance a person working whole day in front of computer and conducting analysis. It requires professional practice and detailed knowledge of psychosocial and biologic dimensions of cancer problems. So it requires thorough practice, learning and research, after this only a person can be p rofessional. While occupation is linked to what one learns in his academic life and implement that in practice, this cannot be considered professionalism. Therefore this role is linked to the definition of professionalism rather than occupation. It is a broader concept then just an occupation (Brant JM & Wickham RS, 2004, p.1). So, the role of oncology nurses focuses on three core areas such as: Patient education Patient assessment Coordination of care Patient education Nurses have more opportunity to expand the knowledge of patients in relation to their disease and to educate them accordingly to have efficiency in treatment. They educate in order to assist patients to deal with their diagnosis, symptoms and long term adjustments, to gain information of prevention, to develop their knowledge and skills or to regain health status. Nurses teach patients through various tools and methods such as visual, printed and audiovisual materials are used by nurses to identify patient needs and abilities. Patient assessment Oncology nurses assess patient’s emotional and physical status, health practices, past history and heath, tries to achieve knowledge in relation to diagnoses from the patient as well as his family. Oncology nurses know the results and implications of pathology, laboratory and imaging studies. Coordination of care Oncology nurses plays imperative role in coordinating the complex and multiple technologies which are used in cancer treatment and diagnoses. This coordination involves taking care of patients, preparing all medical records, management of symptoms, participation of therapy, educating patients as well as family, counseling and follow-ups. Nurses should serve as patient’s first line of communication. Therefore, it is imperative for nurses to get sufficient information about the patient and his family (Johnson Mary, 2004, p. 80). 1.3 Oncology Nursing Education A whole educational curriculum is developed to create understanding and en hance their knowledge of epidemiology, cancer biology, treatment, nursing issues and practices and trends in cancer care. Specific journals which help to

Palmtop Mobile Phones Assignment Example | Topics and Well Written Essays - 4000 words

Palmtop Mobile Phones - Assignment Example It is the world most globalizes country whose capital is London which is the third major financial center of the world. The most recent official figures shows that the GDP growth rate of United Kingdom is 3.1% where as GDP grew in 2006 was 23/4% and more than 3% in 2007. According to 2006 rating, the GDP is $ 2.1 trillion where as commercial service imports increases to $ 600 billion. The population of UK according to 2006 was 60,587,000 and out of this 50,763,000 is living in England. Initially 12 countries were choose by the firm, through screening process out of these 12 countries two countries were left and chosen by us as our product international market. The twelve countries selected by the firm are: International marketing of the product starts by assessing the international market and evaluation of the economic information that directly affect on the firm product [Alan M. Rugman and Richard M. Hodgetts (March 2000)]. Screening process for the product involves series of analysis; therefore, we have divided our screening process into many steps using economic indicators to represent that which two countries will suit best for the product. The initial screening process of the firm requires knowing the basic need and potential of population in international market [Anant K. Sundaram, J. Stewart Black, (1995)]. ... INITIAL SCREENING (Basic need and potential) The initial screening process of the firm requires knowing the basic need and potential of population in international market [Anant K. Sundaram, J. Stewart Black, (1995)]. A simple question "who might be interested in buying our product" is used to answer the basic need of the population. There are three ways to answer these questions 1. Current import policies: the current import policies of Australia, Canada, China, Japan and Germany are strict enough that it can cause a huge cost to our product. The taxes and other expenses on the import of these countries will be high as compared to other countries. As extra cost will incurred on the product that's why the cost of the product will be high in these market, causing less purchase of the PDA. Therefore, the choice of countries will exclude Australia, Canada, China, Japan and Germany. 2. Local production of PDA: Australia, Canada, Japan, China, Germany, Italy and United States of America are producing their own PDA's and therefore, establishing our market will in these market will not provide future establishment as these market already hold strong competitors for our product. China PDA's are now becoming more famous as they are using cheap technology and thus providing low price PDA's in the international market. On the other hand Germany, Italy, Japan and Australia are holding different international companies and national companies producing PDA's. 3. Demographic Changes: the demographic changes in India, Pakistan, Poland and Malaysia are in account and thus the potential for our product in these countries will be high [Lee J. Krajewski and Larry P. Ritzman (2003)]. Where as other countries are developed countries and already hold strong market

Compare and Contrast Herbal medicine and Nutritional therapy Essay

Compare and Contrast Herbal medicine and Nutritional therapy - Essay Example In addition, complementary medicine incorporates alternative medicine applied for curative and therapeutic purposes, instead of the western medicine (Robson, 2003). Other important component of complementary medicine according to Robson, (2003) includes indigenous practices and medicines traditionally used for medical intervention in addition to integrative medicine, which involves using both western medicine and complementary medicine to cure diseases. In view of these dimensions, Robson (2003) argues that complementary medicine is an inclusive term, incorporating both complementary medicines and therapies. In health care, Mark and Brown (2007) note that the major concerns of complementary medicine are maintenance of health and curing diseases. Therefore, different medicines and therapies not regarded by mainstream medical practice are included in the practice. These include herbal medicine, acupuncture, reflexology, aromatherapy, nutritional therapy, hypnotherapy, massage therapy, yoga, homeopathy, osteopathy among others (Mark, & Brown, 2007). According to Blackman, et al. (2009), many health care professionals are increasingly applying both complementary and conventional medicine and therapy in their practices and this has resulted to high incidents of overlap between the two. In this regard, Fass (2001) formulated four domains of complementary medicine considering the existence of some intersections while applying medical practices. These domains include mind and body medicine, practices based on biological applications, energy medicine, and body based (manipulative) practices (Fass, 2001). Mind - body medicine in complementary medicine involves the application of diverse methods intended to improve the power of the mind to affect the functions of the body and symptoms (Damery, et al. 2009). Examples of mind-body

Women In The World War 2 Essay Example | Topics and Well Written Essays - 750 words

Women In The World War 2 - Essay Example This essay aims to provide more insight on the significant role of women during WW2. Moreover, it sheds light on Rosie the Riveter, who as it has been documented in the history of the United States, played a significant role in making sure that women took up the designated jobs and discharged their duties with utmost efficiency. Rosie the Riveter as she is commonly known is an American cultural icon who has overtime been used by historians to symbolize the American women, who formed majority of the work force in American factories when the male employees were in the battlefield. The image of Rosie the Riveter provided encouragement to the women, most of whom took up jobs that they were not trained on, and delivered effectively from the beginning to the end of the war (Williamson 88). The influence of Rosie the Riveter was huge to an extent that at the end of the war in 1945, there were well over 12 million females involved either directly or indirectly in the war. Women played an integral role in the course of WW2, just as it was in the wake of the First World War. In as much as most of them opted for familiar territories by deciding to join the civil service, joining the teaching force and serving as healthcare providers, a considerable number of them plied their trade in several factories throughout the United States. The first and ideally the most important task that women took part in was the evacuation process. This event majorly involved majority of the mature women taking part in evacuating younger women as well as young children from areas that were perceived to be open to war, and taking them to much safer places (Brayley 54). After the evacuation process, it was noted that about 3.5 million children and young women had been evacuated to areas that were regarded as safe. In addition to the evacuation, the women who took part in the evacuation process were tasked with the responsibility of teaching the evacuated children. A

Conflict Coursework Example | Topics and Well Written Essays - 1000 words

Conflict - Coursework Example Relationship conflicts arise between two or more people. It can be between intimate relations or people from due to miscommunication, negative behavior and emotions and stereotype reasons. Larry Alan Nadig who is a family therapist, marriage and clinical psychologist there is no relationship that does not face conflicts. He also says that conflict in a relationship is not necessarily a bad thing. It can teach self-awareness; a person learns about his shortcomings such as communication problems and behavioral and relationship issues. It is often said that conflicts can also make relationships stronger. In a workplace however conflict is more of a negative thing which is why employers try their best to avoid such situations (Boles et al, 2001) According to recent findings conflict at work can also have a positive side (Tjosvold, 1991; Amason and Schweiger, 1994; Jehn, 1994, 1995; Van de Vliert and De Dreu, 1994; Pelled, 1996) for example task-related management team conflict which impr oves growth and overall performance in the organization (Eisenhardt and Schoonhoven, 1990). Data conflicts are raised due to misinformation, insufficiency of information that is required for making the right decision. Sometimes relevance of data is different for different people and so is the way it is collected and communicated. If the data possessed by two different parties is different there is a conflict of data between them. Interest conflicts come into play when two people have different interests because their needs differ from each other. These disputes might be related to issues of money, trust and resources (Thompson, 1993). Conflicts of interest (COI) are basically circumstances forming a risk that a professional decision can be effected by a secondary interest. These conflictscan also be called a conflict of duties. There are five types of conflicts of interest; self-dealing, outside employment, family interests, gifts from friends who are in the same business as them an d pump and dump in which stock brokers inflate prices of a security by spreading rumors about it according to his own interest. Structural conflicts occur due to geographic factors or time constraints. These may also happen because of inadequate physical resources or organizational changes. These types of conflicts have structural solutions if they are mediated in the right way. They are not in the control of the individuals and are caused mainly by external factors therefore solving these conflicts can require the help of external agents meaning anantagonist; a person who are not directly involved in the quarrel. This is the structural conflict theory; the people who have nothing to do with how the conflict started have to help resolve it. There are two types of structural conflicts; symmetrical and complementary schizogenesis. The first kind is one where a lot of negative thoughts and feelings lead to a structural breakdown between the two groups at odds. This leads to an eventual arms race. The second type is the opposite of this situation. Instead of being both parties being hostile the response is more submissive. Surprising as it may seem this scenario tends to escalate a conflict and is more of a compromise rather than being the solution to the problem. The fifth type of conflict is a conflict of values which occurs when individuals form a different group of beliefs or values are in opposition. The clash happens when one person tries to force his belief on the other person. This

Shopping as an American Culture Value Essay Example | Topics and Well Written Essays - 2000 words

Shopping as an American Culture Value - Essay Example The contention is that though American culture has been manipulated into accepting materialistic 'must have' consumerism as a cultural value, there are those, past and present, who provide a glimmer of hope for a return to the better, more humane values of the American way of life. According to Rao (2004), writing from an Indian viewpoint, the American Dream encapsulated "freedom," and "democracy" in a "land of opportunities." In reviewing the book, 'Affluenza: The All Consuming Epidemic', he cited figures from De Graff et al (2003): Embedding of this value was reflected in "one poll found that 93% of teenage American girls rate shopping as their favorite activity." (Rao, 2004). He further contended that only about one quarter of mall shoppers are seeking to buy a specific item, the rest use shopping as therapy, for amusement, or just for its own sake. Americans in general would seem to have adopted shopping as a cultural value, a way of life. ... There is little doubt that people are buying, not from necessity, but spending above their means in order to acquire possessions in a search for happiness and to belong to their culture. They must have the newest fashion, the best brand, the biggest house, the fastest car in order to feel valued. Social theory provides some answers as to how this has happened. In order for businesses to make profits, they no longer seek only to produce to meet needs, but make sure that demand levels stay high, and so maintain the growth of a capitalist system. By marketing and motivating people to buy, this is accomplished; a psychological manipulation appears to be in place. "Advertising, marketing and the mass media have become central to the stimulation of demand through the continual invention of new wants. The images and identities they disseminate promise satisfactions earlier generations never dreamed of. They suggest life-styles of endless acquisition and inexhaustible glamour, which can be had at the pleasurable price of merely buying more and more." (Noble, 2000, p. 231) This shows how people can be sucked into the shopping vortex, with little or no regard for its effects on the individual or the world in general. The impact worldwide, where poorer nations make the goods, on low pay (rendering American workers jobless), in sometimes slave-like conditions, to feed the greed of multinationals and consumers, presents an immoral and inhumane side of capitalism. Sanders (2000), in an article on Maytag and the North American Free Trade Agreement, stated: "The simple truth is that American workers cannot, and should not be "competing" against desperate workers in developing countries who are forced to work for pennies an hour." (Why Overcoming

Microbial Analysis of Soil Essay Example for Free

Microbial Analysis of Soil Essay Abstract: soil samples were collected fortnightly from area near Dahisar River, A river in suburb of Mumbai. laboratory analysis started from July 2010 to September 2010. Total bacterial and fungal count were estimated by standard spread plate isolation. Isolated bacteria were subject to colony characterization and were estimated by their morphological and biochemical characters. As being a monsoon the occurrence of variation of different species were high. The microorganisms isolated from the soil were of staphylococcus strain and were gram positive, aerobic, coccus shaped bacteria. The fungal species were also identified, of which Aspergillus and Penicillium were dominant, followed by mucur, as sub dominant .This project aims to find out the water and soil quality of River and as it is flowing through an industrial area, to find out if it is getting affected by the Industrial pollutants. Introduction: Soil is the region on the earth’s crust where geology and biology meet, the land surface that provides a home to plant animal and microbial life (Pelczar et al., 1993). Soil teems with microscopic life (bacteria, fungi, algae, protozoa and viruses) as well as macroscopic life such as earthworms, nematodes, mites, and insects, and also the root systems of plants. The numbers and kinds of micro- organisms present in soil depend on many environmental factors: amount and type of nutrients available, available moisture, degree of aeration, pH, temperature etc (Prescott et al., 1999). Soil bacteria and fungi play pivotal roles in various biochemical cycles and are responsible for the recycling of organic compounds (Wall and Virginia, 1999). Soil microorganisms also influence above- ground ecosystems by contributing to plant nutrition, plant health, soil structure and soil fertility (O’Donnell et al., 2001). Soil is generally a favorable habitat for the proliferation of microo rganisms, with micro colonies, developing around soil particles. Numbers of micro organism . In soil habitats normally are much higher than those in fresh water or marine habitats (Atals and Bartha, 1998). Bacteria make up the most abundant group of micro- organisms in the soil (3.0 x 106 – 5.0 x 108) per gram of soil, followed by the actinomycetes (1.0 x 106 – 2.0 x 107), fungi (5.0 x 103 – 9.0 x 106), yeast (I.0 x 103 – 1.0 x 106), algae and protozoa (1.0 x 103- 5.0 x 105) and nematodes (50 – 200) counts per gram of soil are wide differences in the relative proportions of individual bacteria genera found in particular soils (Atals and Bartha, 1998). Soil fungi may occur as free-living organisms or in mycorrhizal association with plant roots. Fungi are found primarily in the top 10 cm of the soil and are rarely found below 30 cm. They are most abundant in well-aerated and acidic soils (Domsch et al., 1980). Most fungi in soil are opportunistic (zymogenous). They grow and carry out active metabolism when conditions are favorable which implies adequate moisture, adequate aeration and relatively high concentrations of utilizable substrates (Postage, 1994; Miyanoto et al., 2002). In this research we isolate culturable heterotrophic bacteria and fungi from different top soil samples MATERIALS AND METHODS Laboratory analysis Preparation of materials The materials needed for this experiment include; glass wares (conical flasks, bijou bottles, pipettes, petri-dishes) and they were washed with detergents. These glass wares were rinsed thoroughly with clean distilled portable water and left to air dry before sterilizing them in the autoclave at 15ââ€" ¦C for 1 hour. Also, the laboratory cabinets on which the work would be carried out was swabbed with cotton wool soaked in methylated spirit to sterilize it before any microbiological analysis was carried out to avoid the growth and isolation of other organisms not present in the samples. After sterilization, the plates were allowed to cool to about 45 degrees before they were used. Microbiological evaluation Ten (10) grams of the soil sample for microbiological evaluation was weighed into 9ml of sterile water. Preparation of serial dilution goes thus: 1ml of the original stocks solution was poured into 9ml sterile distilled water and mixed thoroughly to give 10-2 of the original sample and this was done for each sample and the bottles labeled according to date of collection Isolation and Enumeration of Micro-organisms. 1gram of the samples was homogenized in 9mls of distilled water to obtain a ratio of 1:9 and the second diluted of each sample was plated using the pour plate technique. Sterile molten nutrient agar (NA), potato dextrose agar (PDA), macconky’s agar,(MA) manitol salt agar (MSA) and deoxycholate astrate agar (DCA) were used{the potato dextrose agar (PDA) was acidified). These agars were then added and left to solidify undisturbed. These plates were incubated 37oC for 24hours (incubation was aerobic) and the procedure was repeated using 10-2 finally the number of colonies per plates were counted and recorded. The acidified PDA was incubated at 25C for 3-7 days for microbial growth. Total Bacterial counts (Cfu/g) The total bacteria count for each sample was determined with the pour plate techniques using nutrient agar. The plates were incubated between 24hours at 370C and all colonies appearing on the end of the incubation period were counted using digital unlimited colony counter and the counts were expressed in colony forming unit per gram {CFU/g} of the sample. Colonies of bacteria developing on the plates were observed, isolated and reisolated on a fresh media until pure culture was obtained. Preparation of Pure Culture It is necessary to isolate organisms in pure culture before studying and identifying them because a pure culture originates from one cell. Characteristics colonies from the original culture on the plates were picked with a sterile wire loop (using surface streaking method) and this loop was used to make streak of the colony on the surface of newly prepared sterile agar plates of NA,MA MSA. These streak will space out the inoculants and discrete colony of a particular specie of organism and then incubated at 35-37oC for 24hours to enhance microbial growth. Distinct colonies were re-inoculated on another fresh agar plates in order to obtain a pure culture. The isolates were picked with sterile loop and streaked into prepared agar slants, labeled and incubated for growth after which they were kept in the refrigerator for future use and identification. Identification of Isolates These isolated bacteria were identified using both morphological culture characteristics (i.e. the color, shape, elevation, capacity, consistency, edge) and biochemical test (i.e. citrate, oxidase, indole, sugar fermentation, test etc.)and the bacteria were identified based on the results obtained from the above mentioned biochemical characterization results and the procedures include. Grams Staining Techniques A drop of distilled water was placed on a clean glass slide. The inoculating wire loop was sterilized by flaming until it was red hot (this is to prevent the invasion of unwanted micro- organisms that might be inhabiting the wire loop) in the blue flame of a Bunsen burner. The loop was allowed to cool and the small portion of each colony of microorganisms to be gram stained was picked and smeared in the drop of water (distilled) on the glass slide and then spread into a thin smear along the slide. The smear was air dried and passed through the blue flame. The smear was stained with 1%crystal violet and left for 1minutes (60secs) and then washed with running distilled water it was then stained again with Lugols iodine for another 60secs and also washed with running distilled water. The slide was decolorized rapidly with 75% alcohol in order to present the organism from having the color of the primary reagent and it was washed immediately with distilled water. The slide finally was flooded with a counter stain safranine (a secondary stain) for 60secons and also washed off with distilled water and allow to air dry. The slide was covered with a cover slide and observed under the microscope using oil immersion x 100 objective lens with immersion oil. The gram reaction of the isolated arrangement and the shape of the cell were observed and recorded. Gram positive (+ve) bacterial were characterized by a purple color (i.e. the primary stain) while the gram negative (-ve) bacteria were characterized by red color (i.e. the secondary stain) .This procedure is actually used to ascertain the component of each organisms cell wall. Motility Motility was determined by hanging drop techniques. Using loop, a little part of the colony of the organisms were grown in peptone water for 18hours and then placed in the grease free slide and covered with a Vaseline bound cover slip and then observed under x100 objective lens. A motile organism is then seen moving in the drop of liquid. Identification Of Mold Isolates Mold isolated was identified using cultural and morphological characteristics and according to (Fawole and Oso, 2001), microscopic observation was carried out using lacto phenol blue stain. Procedure for Mold Staining A drop of lacto phenol blue stain was dropped on a clean grease free sterilized glass slide and after this a sterile inoculating wire loop was used to pick the mycelium unto the glass slide from the mold culture .The mycelium was spread evenly on the slide. Teasing was carried out to separate the mycelium in order to get a homogenous mixture and the mixture was then covered with cover slips gently and then allowed to stay for some seconds before observing under x40 under the microscope. The microscope examination of actively growing mold was on the basis of structures bearing spores, presence or absence of septate. BIOCHEMICAL TESTS Catalase Test Catalase test demonstrates the presence of catalase enzyme by aerobic microorganisms. Catalase is an enzyme that catalysis the release of oxygen from hydrogen peroxide (H2O2). To test for catalase, a drop of 3% hydrogen peroxide solution was added to a slide and the organism to be tested for catalase production is brought in contact with the hydrogen peroxide. The production of gas bubbles however indicates a positive reaction and this shows that catalase enzyme is produced.(FawoleOso, 2001) Oxidase test This was carried out by placing a clean filter paper on the working bench or petri dishes and 2-3 drops of freshly prepared oxidase reagent was added to the isolate using a sterile inoculating wire loop. After this, a few quantity of oxidase reagent was added and a purple coloration was observed within 10-15minutes which indicated that the organisms is oxidase positive and according to Olutiola et al, 1991, a positive reaction is dependent on the presence of cytochrome. This test is also useful for the separation of Neisseria in mixed culture and in differentiating Pseudomonas from enteric bacteria. Indole test Olutiola et al, 1991, describes the test as one which is important in the differentiation of colonies and it depends on the production of indole from tryptophan by the organism. An inoculating loop was used to inoculate the organism into a test tube containing decarboxylase medium becomes violet. An uninoculated test tube serves as a control (i.e. remained yellow) Sugar fermentation test The ability of the isolates to utilize certain sugar as energy source was tested. If the organism does ferment a particular sugar, acid will be produced and gas may be produced or not. Acid production is indicated by color change of the medium from red to yellow and acid presence could also be detectable with a ph. indicator in the medium while the production of gas is indicated by a void produced in a Durham tube. The fermentation medium was prepared by 0.1g of sodium chloride and 0.1g of fermentable sugar (glucose) in 10ml of distilled water. An amount of 9ml of the medium was pipette into a test tube containing Durham’s tubes in replicates. 5ml of phenol red indicator was immediately discharged into the test tubes. The test tubes containing medium were sterilized in an autoclave at 121 o for 15minutes.After sterilization, each isolate were incubated in glucose Medium. An uninoculated test tube was also incubated for glucose to serve as a control. The test was also carried out using maltose, lactose, galactose, manitol, sucrose, fructose and mannose.(Olutiolaet al., 1991) Discussion: The abundance of bacteria and fungi in this study were typical of environment with high species richness and functional diversity. Despite the fact that it is possible that a number of bacteria and fungi may be missed in this study, the isolates could be readily assigned dominant (e.g. Bacillus sp, Aspergillus sp) or transient/succession roles in the isolation of organisms form different seasons, which form the basis of this study. In additions to the implications of the determination of the number of microorganisms during soil sampling, one should consider the qualitative aspect of the preservation of important species and groups of microorganisms and of the changes in these biochemical characteristics resulting from the variations in these counts. Although the results of this study would not be considered to be exhaustive, as it was done within the limits of facilities available in the laboratory, an insight into the population dynamics and distribution of culturable aerobic bacteria and fungi diversity has been elucidated. This is without prejudice to the possible influence which a substantial proportion of bacteria and fungi that are not culturable in vitro could have on the overall picture of event. It would require more modern technology (nuclei acid probes) to obtain such detailed overview of microbial diversity. This should be a subject of extension of this investigation in future. Conclusion Through this project, if emphasis is made on public health, the observation and findings show striking predominance of Salmonella typhi. And E.coli. E.coli being an enterobacter cause dysentery and S.typhi poses a great risk of typhoid. Health inspector and municipal authorities should look into this matter for further investigation and if possible improvement Acknowledgement Investigators are grateful to the Principal Management of S.V.K.M’s Mithibai College for constant encouragement support. And head of department of zoology Prof. V.V. Dalvie for providing me opportunities and Prof. Radhika D’souza, under whose guidance the project was successfully completed References 1 .Atals RM, Bartha R (1998). Microbial Ecology: Fundamentals and Applications. 4th Edition. Benjamin Cummings Publishing Company Inc. Addison Wesley Longman Inc. pp. 300 – 350. 2. Miyanoto T, Igaraslic T, Takahashi K (2002). Lignin–degradation ability of litter decomposing basidomycetes from picea forest of Hokkaida Myco.sci. (41): 105 – 110. 3. Domsch KH, Gaws W, Anderson TH (1980). Compendium of soil fungi 4. O’ Donnell AG, Seasman M, Macrae A, Waite I, Davies JT (2001). Plants and Fertilizers as drivers of change in microbial community structure and function in soil. Plant Soil (232): 135 – 145. 5. Pelczar MJ, Chan ECS, krieg NR (1993). Microbiology: Concept and Application International edition McGraw-Hill, USA. Pp 281-324. 6. Wall DH, Virginia RA (1999). Controls on soil biodiversity insights from extreme environments. Appl. Soil Ecol. (13): 137–150. 7. Fawole and Oso, 2001

Spectrophotometric Assay for Lipase Activity

Spectrophotometric Assay for Lipase Activity Decomposition of human and animals bodies depends on numbers of factors. One of these factors is the presence of bacteria, both endogenous and exogenous of the body. They use the environmental factors to drive the decomposition of the tissues in the body. The various tissues are degraded at different rates by different bacterial cells. As it was seen in the model burial of a pig that is the early stages of decomposition Gram negative bacterial were mostly present in the decaying body. But after 6 7 weeks later the Gram negative bacteria started to decrease as the number of Gram positive bacteria present in the decaying body started to increase. The bacteria produce enzymes which break down any tissue in the body. In the adipose tissue bacteria produces lipases which is secreted in to he tissue and slowly starts to break down the fat. Lipases producing bacterial has been collected from a model burial environment without any environmental factors to see if there is a difference in the activity of the lipase enzyme which are produced by different bacteria species. These bacteria were used in two of the spectrophotometric assay that has been described in the literature. The turbidity assay shows how quickly the lipase enzyme can break down the lipid in the emulsion solution. On the other hand the BALB (dimercaprol Tributyrate) DTNB (5, 5- dithiobis (2-nitrobenzoic acid)) method shows the increase in the product that is produced by the lipase. INTRODUCTION Lipases are found naturally as it is produced by plants, animals and micro-organisms. In the last few decades, the micro-organism production of lipases has been studied for commercial use, which leads to bacterial lipases being studied a great deal. Lipase enzymes breakdown and mobilize lipids which are present within the cell of the organism and the breakdown of lipid is also present in the environment. However there are many questions still unanswered. For example, is the activity of the lipases different when they are produced by different strains or species of bacteria? Hopefully in this research paper, this question will be answered. Bacterial Lipases When bacteria is grown in a surrounding of hydrophobic media, the bacterial cell releases lipase for the breakdown of fats in the environment for a source of energy. Bacteria produce lipases during the late phases of log phases and in the stationary phases. Lipases are hydrolases which hydrolyzes triacylglycerols in aqueous conditions to form fatty acids and glycerol. The reaction releases energy which is used for growth of the bacteria which is why the bacterium produces lipases within these phases. The substrates of the lipases are triacylglycerols which are hydrophobic and the reaction occurs in aqueous condition and this leads to the reaction occurring in lipid-water interface. Some lipases can also catalyze the synthesis of long chain fatty acids. Lipases contains ÃŽ ±/ÃŽ ² fold, which has eight ÃŽ ² sheets in the middle which are parallel except for the second ÃŽ ² sheet and the sheets are surrounded by ÃŽ ± helices. This fold offers a scaffold for the active site in the lipase molecule. The active site or binding site of the lipase molecule is where the interface occurs. This is where the chains of the enzyme are subdivided; at the bottom of the active site is where the ester bond binds to which means this region is hydrophilic. Towards the surface of the enzyme is where the molecule binds to the fatty acids and therefore this region is hydrophobic. Within the ÃŽ ²-sheets there is an area which is highly conserved which is made up of the triad which is a nucleophile and histidine. The nucleophile is made up several amino acids, which are Serine, Cysteine or aspartic acid. The nucleophile is present on ÃŽ ²5 and the histidine is present on ÃŽ ²7. The histidine is the only highly conserved area of the active site/enzyme that d iffers in shape and structure from one type of lipase enzyme to another. Another area of the active site that is important but only present in some type of lipases is the lid. This area is what gives the lipase enzyme the structural explanation of the interface property. When the substrate comes into contract with the lid, it opens the lipid water interface where the substrate binds to for the reaction to occur. Some lipase molecules are only active in the presence of Ca2+ and this is due to the subdivisions of the active site being bound together by the Ca2+ion. The hydrophobic region of the active site leads to less inhibitors that can bind to and inactivate the enzyme. Since lipases are extracellular enzymes, the secretion/production of these enzymes is affected by a number of factors: Nutritional enzymes are produced when the bacteria is in the presence of a lipid environment such as oil, tweens, hydrolyzable esters and triacylglycerols. These are the main sources of lipid but many bacteria can produce lipases in the presence of various sources of substrates. For example Pseudomonas aeruginosa produce lipase in the presence of long chain fatty acids such as oleic and linoleic acid. Temperature the temperature at which maximum production of lipase can occur depends on the optimum temperature for growth of bacteria. The temperature normally ranges from 30 60Â °C, but some can survive at colder or warmer temperatures. Therefore it depends on the type of bacteria in question. pH normally bacterial lipases are active in neutral pH or alkaline pH. However there are a few exceptions like Pseudomonas fluorescens lipase has an optimum pH of 4.8, whereas most bacterial species possess stability over a broad range of pH of 4 10. Effect of ion one type of lipase which is produced by Pseudomonas species is activated by the presence of Ca2+ ion in the environment. Growth of bacteria if the bacterial cell is present in the log phase then the production of lipase is decreased in the bacterial cell. Inhibitors inhibition of lipases does not affect the production or the secretion of the enzyme but affects the activity of the enzyme. There are two types of inhibitors; irreversible or reversible. The reversible inhibitors are split into two types. The first of which are non specific as they bind to the enzyme but not at the active site. When the inhibitor binds to the enzyme, the active site changes and therefore prevents the lipases from binding to the substrate as the structure of the active site has been changed. An example of this type of inhibitor is bile salts. However bile salts can activate some lipases such as the lipase produced by the pancreas. The second type of reversible inhibitors is specific inhibitors as they bind to the active site of the lipase enzyme. They can also be irreversible as the interaction between the inhibitor and the enzyme is so strong that it cannot be broken. An example of this type of inhibitor is boronic acid which can bind to the active site f or a long time but can still be removes leaving the active site unchanged. These types of inhibitors bind to the triad of the active site, which means that when they bind to the triad, the interaction is irreversible. There are three major types of microbial lipases depending on the substrate they bind to. Nonspecific these enzymes act randomly on the lipid substrate molecules which then completely breakdown the molecule. For example with the triglyceride molecule, the enzyme will break the ester in random fashion until the molecule is complete broken down to fatty acids and glycerol. Regiospecific these enzymes only hydrolyze the primary ester bond, these are the C1 and C3 bonds in the triglyceride molecule , which means that when hydrolyzing triglycerides the final products are free fatty acids, 1, 2(2,3)-diacylglyceride and 2-monoacylglyceride. Fatty acid-specific there are some bacteria that only produce this type of lipase and they bind to fatty acids which are then broken down by the lipase. One type of bacteria that can produce lipases that only bind fatty acids is the Achromobacterium lipolyticum. Other bacteria that produce this type of enzyme are Bacillus species which mostly bind to long chained fatty acids. However other bacteria like Pseudomonas species produce lipases that can bind to short or medium length of fatty acids. Staphylococcus aureus can produce a lipase molecule that can bind to unsaturated fatty acids. Lipase in Decomposition The bacteria that are going to be used in the research project are bacteria that were purified from a model burial environment. The bacteria that were present in the model burial environment must have been already been present in the pigs body, which means that all the bacteria that are going to be used are endogenous bacteria that are part of the pigs microflora. The bacteria sample had been taken out of the fluid from the decaying organism in a steel box which was free from all external environmental factors except from oxygen. The sample of bacteria was taken two times a week and then towards the end it was reduced to once a week. It was discovered that at the beginning of the decaying process the bacteria that were present were Gram negative bacteria. However after week 9 the bacteria that were growing in the decaying pig changed from Gram negative to Gram positive. These bacterial cells can release lipases which can break down fats in the body which leads to the formation of adi pocere. Adipocere is made up from a mixture of saturated fatty acids which have been produced during decomposition of the adipose tissue in the body. These adipoceres are formed straight away after death by lipases which are present inside the body. These lipases are mostly produced by the bacteria in the body of the pig which breaks down triglycerides to free fatty acids. If in a suitable environment, bacteria release lipases for hydrogenation of unsaturated fatty acids to its saturated form. Lipase Assays There are two assays that will be performed to find out the activity of the lipase which are present in the solution. The first is based on BALB DTNB method and it uses dimercaprol tributyrate (BALB) and 5, 5 dithiobis (2-nitrobenzoic acid) (DNTB). The lipase enzyme binds to BALB and cleaves it to form an SH group which then binds to DNTB. The product then forms a yellow product which then increases the absorbance which can be measured using a spectrophotometer. The colour intensity is measured at 412 nm; the colour change is proportional to the activity to lipase at to 1:1 ratio. The second assay also uses the spectrophotometer but this time it measures the optical density of the solution instead of measuring the amount of product that is formed. Tributyrin and olive oil is emulsified in the solution which gives a turbid appearance. As the lipase breaks down the lipid in the assay solution, the optical density of the solution decreases which can be measured. The optical density of the solute ion can be measured at 450nm. Both assays measure the activity of the lipase but in two different ways. The first measures the amount of product that is formed while the second measures the breakdown of the substrate. AIMS AND OBJECTIVES Decomposition of human or animal bodies is dependent upon a number of factors. Bacteria which are endogenous (in the body) and exogenous (in the environment) are the key components of decomposition. Different tissues in the body degrade at different rates and are degraded by different bacteria. Previously it has been shown that bacteria in the model burial environment can produce lipases which breakdown the lipids found within the tissues of the body. However it does not tell you if there are different lipases that are secreted by different bacterial cells. Lipase production was demonstrated by using plate assay when lipase breaks down tween 20. Therefore it does not compare the different lipases produced and the activity of different bacterial species. There have been different spectrophotometric assays that have been described in the literature to calculate the activity of lipase enzymes, but only two of these will be used. The bacteria that is going to be used in the assay has been purified from fluid from a decaying pig in a steel box which is free from all external environmental factors expect oxygen. Two assays are going to be preformed to find the activity of lipase, the first one similar to the BALB DTNB method. Lipase forms a SH group on BALB which then binds to DTNM to give a yellow product. The amount of product that is formed in a solution is related to the activity of lipase in a 1:1 reacting ratio which is a direct measurement of the activity. The colour change is measured at 420 nm. The second assay is also measure the change in the solution but this time it measures the decrease of the substrate that is left in the solution. It measures the density of the solution, as the substrate (olive oil) is denser than the product. The density is measured 450 nm. The decreased of the substrate is related to the activity of lipase. At first before anything can be done we need to see if the bacteria cells produced lipase is by growing them in a plate which contains Tween 80. If the Tween is broken down then the bacterial cell produces lipase. MATERIALS AND METHODS The bacterial strains that were given to me were extracted from fluid from a pig that was decaying in a steel box which had a controlled environment that was free from all external environment factors expect fresh air. Bacterial Media The bacterial strains were grown in half nutrient agar which was made from 2.6g of nutrient broth (OXOID, Basingstoke, England) and 4.8g of Agar bacteriological (OXIOD) in 400ml of water which was autoclaved and then poured in to 20ml Petri dish. The bacterial strains were plated and left in a 30Â °C incubator overnight. After the bacteria were grown on just half nutrient agar, they were then grown on half nutrient agar with 4ml of sterile Tween 80 (SIGMA ALDRICH, UK) and 400Â µl of 10% of CaCl2 (scientific equipment, Loughborough, England). Again the plates were placed in a 30Â °C incubator. The bacterial strains were also grown in minimal medium agar which contained 2.8g of Potassium Hydrogen Orthophosphate (BDH Laboratory Supplies, Poole, England), 1.2g Sodium Dihydrogen Orthophosphate (BDH LS) and 0.04g of Magnesium Sulphate (BDH LS) in 200ml of sterile water and 2.4g of Agar bacteriological. After the solution came out of the autoclave, 2ml of Tween 80 was added and 200Â µl of 10 % CaCl2. For the bacterial strains to be used in spectrophotometric assay, the strains had to be grown in liquid media. The bacterial strains were grown in two different types of media, Tryptic Soy Broth and Minimal Medium. The Tryptic Soy Broth (TBS) was made from 30g/L Tryptone Soya Broth (OXIOD) which was autoclaved. After the bacteria were added to the media, the bottle was placed in a shaking incubator at 37Â °C over night. The Minimal Medium contained 14g/L of potassium hydrogen orthophosphate, 6g/L sodium dihydrogen orthophosphate and 0.2g/L of magnesium sulphate. 100Â µl of Tributyrate (SIGMA ALDRICH) was added to 10ml of the Minimal Media. The bacteria were added to the media and then placed in a shaking incubator at 37Â °C over night. Sample Solutions After the bacteria are left to grow, the media is used to make up three different samples of bacteria to use in both of the assays. The first sample is purified bacterial strain from the media and this was obtained when 1ml of the media was placed in a sterile eppendorf tube which was then centrifuged at full speed for 2 minutes. The supernatant was replaced with 500Â µl of 150mM of CaCl2 and 500Â µl of 200mM of Tris buffer (12.11g of Trizma base in 150ml of water and then 0.1M of HCl was added to make the pH of the solution 8, this to make 0.5M Tris Buffer which was then diluted to make 200mM solution) (SIGAM ALDICH). The second sample was done in the same manner but instead of adding Tris buffer and CaCl2 to the pellet, PBS (Phosphate buffered saline) solution is utilized to re-suspend the pellet and 2ml of the media solution is used. Each suspension was transferred in to a different Bijou Bottle which is kept on ice. The suspension in the Bijou Bottle is sonicated twice for 30 seconds at 30W. The last sample was made when the media solution is filtered with the use of a sterile syringe and sterile 0.2Â µm pore syringe filter and placing the filtered solution into a sterile universal bottle. 3ml of the media was only filtered. The samples were ready for the assay and two different that were used. They both measured the absorbance of the solution at different wavelengths. One measured the turbidity of the solution while the other looked at the change in the absorbance of the solution. Turbidity Assay For the turbidity assay an emulsion solution is made and it is made from 100mM of Tris buffer (4.975ml), 50mM of CaCl2 (4.975ml) and 50ml of lipid source (either olive oil or Tributyrate or both). The solution was sonicated for 3 minutes at 40W. The solution is left in a water bath until it is used for the assay. The emulsion solution is used in three different ways as the assay was performed in a cuvette, Petri dish or 96 well plate. When done in a cuvette, 40mg of low melting point agarose (SIGMA ALDRICH) is added and the boiled before sonication. The agarose stabilises the emulsion. If the assay was done in a 96 well plate, then no agarose is necessary. The last test that is performed is in 20ml plates; 20ml of the emulsion solution is made up with 80mg of agarose to made a solid media (INVITROGEN, Paisley, UK) which is then boiled before and after sonication. For the 96 wells plate, 200Â µl of the emulsion solution was placed in each well and then 20Â µl of the sample solution was added. As soon as the sample was added the absorbance is measured at 450nm to measure the optical density of the solution. The absorbance was then measured every 15 minutes up to 60 minutes. Here the samples that were used were grown in the Minimal Medium. The lipid source in this part of the assay was 25Â µl of olive oil and 25Â µl of Tributyrate in 10ml of the emulsion solution. For the assay that was done in the cuvette 1L of the emulsion solution was added to a micro cuvette and 100Â µl of the sample solution. The absorbance was also measured at 450nm as soon as the sample is been added and then every 5 minutes up to 45 minutes. The lipid source is 50Â µl of olive oil in 10ml of emulsion solution. For the plate assay after the solution was boiled for the second time, the solution was poured in to a plate for the agarose to set. After the agarose was set, wells were made in the agarose using a hollow punch about 8mm in diameter which was filled with 10Â µl of the sample solution and the plate was left at room temperature over night. In 20ml of the emulsion solution the lipid source was 50Â µl of each olive oil and Tributyrate. Colour Assay (BALB DNTB Method) The second assay measures the absorbance change in the working solution. The working solution is made from BALB (SIGMA ALDRICH) and DNTB (SIGMA ALDRICH) and Tris buffer solution. The working solution was made from 1 ml of BALB is added to 17.5ml of 0.5M of Tris Buffer at pH 8.5 and 625mg of DNTB. 150Â µl of the working solution is added to the well after adding 150Â µl of water. To this 10Â µl of the sample was added. When the assay was done in 96 well plate the absorbance was measured after the sample was added at 405nm and then every 10 minutes for 30 minutes. When the assay was done in a cuvette, at first 400Â µl of water was placed in the cuvette then 380Â µl of the working solution was added to the water. Then the 20Â µl of the working sample was added into the cuvette. The absorbance was the measured at 412 nm for the 20 minutes. The reason why there is a difference in the wavelength in which the absorbance is measured is due to the plate reader not being able to read the absorbance at 412nm. For this assay the samples that were used were prepared from the bacteria that were grown in TSB. RESULTS When the bacteria colonies were grown on the agar plate which had Tween 80 and CaCl2, around the colonies there was the presence of halos or the colonies has a halo this can be seen in figure 1a. The arrow shows the halo colonies of the bacteria species. The bacteria colonies that were placed on other plates was not as clear as 16C but the halo can only be seen when the plates are held up by the light (result not shown). Turbidity Assay The first assay that was done was the turbidity assay in a cuvette, the optical density of the solution did not increase or decrease, and it just stayed the same. But when the assay was done in the 96 well plate the optical density increased when the bacteria were added to the well, and then decrease and keep decreasing even after 60 minutes (figure 2a). Then the filtered media was added to the emulsion solution in the 96 well plate, the optical density again decreased. However not all the bacteria were filtered to see if there was a decrease in the optical density (figure 3). Only some of the bacteria were used to see if it was an enzyme that was decreasing the optical density and not the bacterial cells. However the general result showed a decrease in the optical density except for 2 bacterial strains (1A and 4A) which showed an increase in the optical density after 30 minutes and then it optical density again. Then the bacteria cell free lysates were added to the welled plate and the same result appeared as the optical density levels decreased once again. The bacteria that were used were the same bacteria that were used in the filtered part of the assay (figure 4). After 45 minutes the optical density is starting to level off. The gradient of the line for all the bacteria strains are the same as they all decrease at the same rate expect for bacteria strain 5 which has flatter gradient than the rest. For the plate test in the turbidity assay, the bacterial solution in the well was not present and no zone of clearance was noticeable in any of the plates (figure 1b). Only one of the plates is shown in the figure and the rest of the plates looked the same as no zone could be seen. Colour Assay (BALB DNTB Method) In the BALB-DNTB method, the absorbance increases when bacteria strain 6 was added to the working solution in a cuvette and measured for 20 minutes. The increase was slow for the first 10 minutes and then increased at a faster rate for the next 10 minutes, figure 5. When the assay was done in the welled plate, the absorbance increases for all the strains but some increase more than others. For example strain 5 increased from 4.204 to 4.412 while strain 1 only increased from 4.241 to 4.265. This is shown in a table in figure 2b. When only the media in which the bacteria grew in was added as the sample, the absorbance also increased for most of the bacterial strains but not as much as when the bacterial cells were added. For some of the strains the absorbance decreased. For example in strain 1 there was a decrease from 4.241 to 4.235, figure 2c. The same happened when the content of the bacterial cell was added to the working solution. But when the absorbance increased, the increase was bigger than the increase when media was added (figure 2d). However there were still some strains in which the absorbance still decreased in 20 minutes but the absorbance increased from 0 to 10 minutes and then decreased from 10 to 20 minutes. Figure 1, (a) the plate has been plated with strain 16C (left) and 16B (right); the halo can be seen clear by the arrow which is the colonies of bacteria 16C. However the halo can not be seen clearly in the colonies of bacteria. (b), the plate contain solid emulsion solution with well which contain lipases from different bacteria, and there is no presence of zone of clearance from any of the well. There were 3 plates in total and all look the same (only one is shown) but the well had different lipases from different bacteria. Figure 2, A is a table that shows the optical density change when bacterial was added to emulsion solution for the turbidity assay. The optical density decreases when the bacterial cells were added to the emulsion solution. The next 3 tables are showing the absorbance change when the strains were added to the working solution for the colour BALB-DNTB method, (B) has bacterial cells added to the working solution; (C) has only filtered media, which had bacteria growing in, was added and lastly (D) had bacterial cells free lysates added. In the colour assay the absorbance increased in all three cases. DISCUSSION Bacteria produce lipases that can break down or hydrolyse lipid molecules such as fats and oils. They produce lipases in the log phase of growth when there is a high level of lipid source for energy. There are different lipases which can break down different lipid molecules. The bacterium produces lipases to break down lipid for energy as adequate amount energy is present in lipids. As most of the lipids cannot cross the cell membrane, the lipid has to be catabolised into smaller lipid molecules which can then enter the cell where it is broken down further. Lipases from bacteria are studied for industrial uses. Here it was studied to see if the lipases that were produced from different bacteria are different and if there was any variation in the activity of the lipases. When the bacterial cells were grown on agar plate without any Tween 80 the bacterial colonies do not have any halos or precipitate around the colonies. But when some of the bacteria were grown in agar that contained Tween 80 and CaCl2 the colonies had halo colonies 3 to 8 days after they were inoculated. In the past Tween has been used for lipase activity to see if the bacteria produce lipase. If lipases are produced then it binds to the Tween and breaks the Tween down to fatty acids. The fatty acids then bind to the Ca in the media which forms crystals. These crystals then become soluble in the media which can then be seen by eye as halos. Some of the colonies had halos which meant that the cell produced lipases. Figure 6, the turbidity plate assay should have looked like this but what the figure 1b shows. There the one of clearance can be seen very clearly where as in the plate in figure 1b there are no clearing at all what meant the assay did not work at all. The turbidity assay that was done is the plate which showed no zone of clearance, it should have had zone of clearance around the well which contained the sample of bacteria. The bacteria in the wells should have diffused out of the well and in to the agarose media in which the bacteria should have released lipases to break down the olive oil and Tributyrate. When the lipids were broken down the media would have become clear. The plate should have look like figure 6 from, the zone of clearance is shown very clearly. The other assay that did not work was the same assay that was done with the cuvette. This is when the absorbance levels did not decrease but just stayed the same. The absorbance levels should have decreased and the reason in why this did not occur is not known. It might have been due to the stability of the solution as the agarose must have been concentrated which meant that the bacteria solution was not able to diffuse through the media. The concentration of agarose might be the problem because when agarose was not added like in the 96 well plate part of the assay, the absorbance of the emulsion solution decreased. This was due to the emulsion solution being turbid by lipid in the solution when sonicated, when the bacteria sample was added the optical density increased slightly as the bacteria cell scatter the light which leads to the increase in the optical density absorbance levels. The bacteria cell then releases lipase in the solution or lipase that are inside the cell break down the lipid in the emulsion solution which then leads to the decrease in the level of lipid in the emulsion solution which then means that less light is scattered. The well plate assay was done to 3 different type of sample solution, one of which contained bacteria cell, one of which contained the filtered media solution and the last contained the bacteria cell free lysates. The bacterial cells were used to see if the bacterial cell produced lipases. The filtered media was used to see if the bacterial cell released lipase in to the media and if it was in fact the lipase that was decreasing the absorbance and not anything else. The bacteria content was used after the bacteria cell were sonicated for one minute, to use all the lipases that had been produced by the bacterial cell but not secreted. As not all the bacteria cells release the lipase in to the media and sometime the lipid molecule is too big to cross the cell membrane and wall of the bacteria. To see if there are any differences in the activity of the different lipases which are produced by different bacterial cells, cannot be done by adding the sample to the emulsion solution as different concentration of lipase must have been in the sample for each of the strains. In order to make the test fair, the amount of bacterial cell and the lipase concentration must be the same for each of the bacterial strain. But still it might be a fair test as some of the bacterial cells can still divide inside the emulsion solution and then increase the concentration of lipases. The lipases produced by the bacteria are produced in the log phase. The same can be said for the BALB-DNTB method. This assay is not like the other assay because the absorbance does not decrease but increase. This is due to the lipase bind to the BALB in which is cleaved to form a SH group. The SH group then binds to DNTB which is in excess in the working solution, to form a yellow substance. The complex then absorbs light hence increasing the level of absorbance. The bind of the BALB with the new SH group binds to the DNTB in a one to one reacting ratio, this means that increases is absorbance is proportional to the reacting activity of the lipase. When bacterial cells were mixed to the working solution the absorbance for most of them increase. This meant that lipases that were present in the well were cleaved BALB. The same thing also occurred when filtered media was added to the working solution but the increase were small and this must be due to the fact that not a lot of lipases were released by the bacterial cells in to the media solution. However, when the bacterial cell free lysates is added not all of the absorbance levels increase but in fact some of them decrease and then increase. It may mean that the lipases need time to start working since they had been on ice before the experiment. To see if this was true, the test needs to be done again but for a longer period of time. In the cuvette test, only one strain, it was used when the first assay was done it had the largest change in absorbance. It was used to see a general increase of the solution over 20 minutes and the absorbance was measured every minute to see the turning point when the rate of enzymatic activity change from being slow to a steady normal rate. The graph in figure 5 shows that the rate was slow during the first 10 minute this meant the bacteria cell needed to adapt to the new environment before the activity of the enzyme can to back to normal. If the test was done longer then the graph would start to level due to the substrate concentration starting to decrease. From the results, there is not enough evidence to conclude that there any differences in the activity of the different strains of lipase. To see if it is true then the both of the a

Symbolic Illustration of the Power of Relationships in Susan Glaspells

Symbolic Illustration of the Power of Relationships in Susan Glaspell's Trifles A friend can be a remarkable thing. Unfortunately, many lack the powerful bonds that all humans need to survive and lead healthy, happy lives. In Susan Glaspell's play Trifles, Mrs. Wright is starved of the human interaction and relationships she so desperately needs. Consequently, she is never rescued from her loneliness, is brought to the point where she cannot handle any more of life's saddening struggles, and kills her husband in his sleep. Through powerful and often ironic symbolism, such as Mrs. Wright's kitchen, the names of the characters, and the bird, Susan Glaspell clearly displays the power of human relationships and how truly devastating a lack of this absolute necessity can be. One of the numerous symbols Glaspell uses to emphasize the importance of wholesome human relationships is Mrs. Wright's kitchen. Upon entering the crime scene, the men and women notice the unkept kitchen. They are alarmed by the "Dirty towels" (Glaspell 1174), the unwashed pans under the sink, a loaf of bread outside the breadbox," "the walls covered with a faded wall paper" (Glaspell 1172), and the "sticky" shelves (Glaspell 1174). The abrupt, incomplete work reflects the emptiness Mrs. Wright had bottled inside of herself and also displays the sudden sense of explosion she must have experienced to go as far as murdering Mr. Wright. Also, they see a small chair beside the kitchen table. Obviously intended for a child, the small chair illustrates Mrs. Wright's empty expectations of raising children. Mrs. Hale explains, "Not having children makes less work-but it makes a quiet house, and Wright out to work all day, and no company when he did come in" (Glas... ...there are so many that go unnoticed and unappreciated. Unfortunately, they do not know how to reach out for help until it is too late. There are also many others that see these lonely and depressed individuals, but no one ever does. Mrs. Peters explains regretfully, "Somehow we just don't see how it is with other folks until-something turns up" (Glaspell 1178). Many times, it is unfortunately too late to save a person. Through her powerful symbols, Glaspell stresses the importance of reaching out to those that are lonely and need emotional support before it is too late. After all, "We all go through the same things-it's all just a different kind of same thing" (Glaspell 1180). Work Cited Glaspell, Susan. Trifles. The Bedford Introduction to Literature: Reading, Thinking, Writing. 5th ed. Ed. Michael Meyer. Boston: Bedford/St. Martin's, 1999. 1172-1181.

CJD Disease :: essays research papers

CJD Disease   Ã‚  Ã‚  Ã‚  Ã‚  The Creutzfeld-Jakob Disease is a rare brain disorder that is fatal. Reseachers find about one case of CJD disease per million each year.CJD can effect anyone, this disease effects both males and females of different ethnic groups usually between the ages of 50-75 .This disease causes progressive dementia and neuromuscular problems. Researchers still don’t know for sure what agent causes the Creutzfeld-Jakob Disease, it is a topic that has been debated about. It was first thought to be a virus but a virus contains nucleic acid and when researchers looked at the CJD agent, they found it contained no nucleic acid. Also the chemicals that are supposed to make most viruses inactive did nothing to decrease the inefficiency of the CJD disease. There is a new theory thought that seems to make more sense. The theory that the CJD disease is not a virus but an uncoventional agent made of protein. This pathogen called a â€Å"prion â€Å" are thought to transform other protein molecules into deadly ones by changing the shape of the healthy molecules to the dangerous conformation. Prions are what link CJD disease and BSE disease. Bovine spongiform encephalopathy, also know as â€Å"mad cow disease,† is believe to be caused by prions, which is believed to cause CJD disease in humans.   Ã‚  Ã‚  Ã‚  Ã‚  Creutzfeldt-jakob disease can acquired in three ways. First, The disease can occur sporadically, this is when there is no evidence of the disease in the person’s family. Most CJD cases occur sporadically, so it’s hard to find the orgins. Second, the disease can be caused by inheritance. This is when someone shows a mutation in the gene coding for a prion protein that was passed to them by genetics. The third way of aquiring this disease is through infection. Researchers are not sure if this is a true way to get the disease but some doctors have gotten the disease after being expose to the infectious material inside someone who had the disease.   Ã‚  Ã‚  Ã‚  Ã‚  There are many symptoms of Creutzfeldt-Jakob Disease as it goes on. The average time between symptoms and inevitable death is usually around one year. One symptom of the disease is a bad case of insomnia. Other symptoms include depression, confusion, personality and behavioral changes, strange physical sensations, and problems with memory, coordination and sight. As it goes on, the person develops dementia in most cases and develops irregular jerking movements.

Reaction Paper Related on Business Communication Essay

Further, to fully use new pedagogical possibilities offered by ICT, profound changes in managers’ conceptions of learning and knowledge are required. Technical expertise alone is not sufficient for exploiting new pedagogical possibilities provided by ICT; insofar as ICT is used in the educational system as a purely technical innovation, it is not likely that significant pedagogical progress will be achieved. Several cognitive researchers (e. g. , Salomon, 1997; Salomon ; Perkins, 1996; Scardamalia ; Bereiter, 1994) have pointed out that many applications of educational technology support only lower-level processing of knowledge. Yet new pedagogical models of using educational technology, and particularly computer-supported collaborative learning environments, promise to provide new opportunities for solving pedagogical problems in the schools. Scardamalia and Bereiter (1994; in press), and others, have proposed that to meet the future challenges, schools be transformed into communities where productive working for advancing communal knowledge is a primary goal of both students and managers. Knowledge building refers to a process of advancing understanding by setting up, articulating, and answering research questions, searching and exploring information, and generating and evaluating explanations. In the present study, the sustained processes of advancing and building of knowledge characteristic of scientific inquiry and knowledge-creating organizations are called â€Å"progressive inquiry. † Several, concurrent, cognitive research projects share a common goal of fostering such research-like processes of inquiry in education.

Dbq Indian Removal Essay

DBQ When the Native Americans lived east of the Mississippi river, they didn’t want to follow the law and be part of U.S. government and wanted to govern their own people. Andrew Jackson being a president of United States didn’t want the group to ignore the government therefore, proposed to move them west of the Mississippi river. In order to justify and keep threats away from the U.S. settlers, the U.S. government promised them bigger land, money, pay for their needs and support for one year as said in the excerpt from Indian Removal Act 1830 (source 1). This act should be justified because it resolved the conflicts between the U.S. and the Indies were given comparable land and support. In Source 2 Andrew Jackson makes a speech about if the Indians movie it will benefit the U.S. and make Alabama and Mississippi stronger. The U.S. wanted to separate the Indians for many reasons. Andrew Jackson wanted the Indians to stay away from whites, â€Å"by opening the whole territory between the Tennessee on the north Louisiana on the south to the settlement of the whites will incalculably strengthen the southwest frontier and render the adjustment States strong enough to repel future invasion without remote aid†( article 2). Also the government thought if they separated the Indian it will enable them to stay away from whites and convince them from their own savage habits and make them more interesting and important. When the government told the Indians to move, they said they would give them bigger land, money and support to move. â€Å"The Cherokee Nations cedes to the United States all the land claimed by said Nation east of the Mississippi River†¦ 7,000,000 acres of land is guaranteed to the Cherokees west of the Mississippi† (source 5). The United States as well, â€Å"agreed that the land herein guaranteed to the Cherokees shall never, without their consent, be included within†¦any State or Territory† (source 5). As the Indians were getting ready to leave, the Americans took away â€Å"all laws†¦ and regulations†¦enacted by the Cherokee Indians†¦ are hereby declared to be null and void and no effect, as if the same had never existed.†(source 3). Organizer Details/ evidence * Paragraph 2 * The U.S. wanted to separate the Indians for many reasons. * Andrew Jackson wanted the Indians to stay away from whites * â€Å"By opening the whole territory between the Tennessee on the north Louisiana on the south to the settlement of the whites will incalculably strengthen the southwest frontier and render the adjustment States strong enough to repel future invasion without remote aid† (article 2). * the government thought if they separated the Indian it will enable them to stay away from whites and convince them from their own savage habits and make them more interesting and important. * Paragraph 3 * When the government told the Indians to move, they said they would give them bigger land, money and support to move. * â€Å"The Cherokee Nations cedes to the United States all the land claimed by said Nation east of the Mississippi River†¦ 7,000,000 acres of land is guaranteed to the Cherokees west of the Mississippi† (source 5). * The United States as well, â€Å"agreed that the land herein guaranteed to the Cherokees shall never, without their consent, be included within†¦any State or Territory† (source 5). * As the Indians were getting ready to leave, the Americans took away â€Å"all laws†¦ and regulations†¦enacted by the Cherokee Indians†¦ are hereby declared to be null and void and no effect, as if the same had never existed.†(Source 3).

Women in the War

1. In Britain in 1914 and before, women were thought of as second class citizens. Women had few privileges that men had; they were down upon by men. Women's employment opportunities were limited and their pay was considerably less than a man's. All this was due to strong discrimination about women being of less importance and intelligence, the general view was traditional one which inferred that women should be housewives. Only one third of women were in paid employment. However there were differences between the jobs they did because of their class. Middle class and Working class women did very different jobs. Working class women worked in more manual, and labour intensive jobs. Whilst Middle class did more intellectual jobs. So we already know that there were clear distinctions between the jobs the women of different classes did. Working class women mostly did domestic services such as cleaning or being servants for the rich. They had to work in poor conditions and were subjected to long working hours. On top of this they received criticism, low wages and got little time off. A major employer was the textiles industry in which women could supervise, yet men often get these jobs. Women also made clothes and dresses, or jewellery or painted ornaments. Middle class women experienced better working conditions. These women were more likely to work as teachers, nurses, secretaries and shop assistants. Women had no political vote and were looked down on as inferior to men. Before 1914 jobs for women were limited and discouraged due to traditional beliefs about the role of women. Within this discrimination there was further discrimination between the classes of women. They were expected to manage the house. People were aware of this and a group called the Suffragettes voiced the opinion that equality should be imposed. This all changed when war broke out. 2. When war broke out the men went to war, this meant that they left their vacant jobs behind. The country was behind the war effort and all came together. Women were at first not allowed to fill the men's jobs, they were only allowed to knit and fundraise. People, including Emmeline Pankhurst, a leading Suffragette realised that women could help more. Pankhurst in July 1915 organised a ‘Right to Serve' march in which 60 000 women took part. There was also an increasing demand for shells due to shortages on the front line. Lloyd George, the Minister of Munitions had to negotiate with trade unions to let women work. They came to a deal known as the ‘Treasury Agreements'. Women began to work in industrial employment; they began manufacturing munitions and shells. The government backed Pankhurst further by giving them à ¯Ã‚ ¿Ã‚ ½3000 to organise processions. Also the women war register was established; it contained all the names of women wanting to help. Also more jobs needed filling when, in early 1916 the government introduced conscription as they realised they were in for the long slog. The war wouldn't be over any time soon. Vigorous campaigning ensued with extensive propaganda encouraging women to work instead of men in industry, farming and the armed services. As a result of increased levels of women working birth rates were falling, this was because women were worried about raising children during wartime. So to ease worries, the government increased the number of child welfare centres so that children and babies had a place where they could be cared for. Female employment levels rose massively due to encouragement, campaigning and thanks to the women's will to help the country win the war. Another reason is because men had to go fight on the frontlines so in order to keep up the production of munitions and shells, the women had to fill in the men's jobs. 3. Before the war women were limited to working in textiles even though they were paid at a fraction of the money that men were. Only a third of women were in paid employment. There were strict traditional rules in society which made it clear that some jobs were purely a certain gender. Women of a lower class had to generally work as domestic services for the rich and middle class women worked doing clerical work and teaching. Women were seen as 2nd class citizens. When the war broke out the men left to go to war meaning that there were vacant jobs that needed to be filled. At first the government were reluctant, but later they realised that women could make a big difference. Protests organised by the Suffragettes encouraged women to work. Women worked in industry, medical and many new areas of employment now. Women though were still treated badly, underpaid and overworked. Some men resented the women and say them as inferior. More positively though is that women became freer and some women over 30 could vote. After 4 years of war, it was over; the allies had won and the men returned home. Women were pressured to leave their jobs for the men and go back to their old jobs, mostly housekeeping. Women did leave work and female employment levels returned to what they were before 1914. The jobs that women worked in changed slightly though as more women worked in areas such as law and medicine, pay did also improve. In the short term it looked like not much had changed, things were back to normal. Women were still paid less and weren't promoted above men. However in the long run World War 1 changed the role of women and had a massive impact as they earned the respect and privileges that they deserved for their contributions. It had been made clear that women were capable of many things that men could do and over time the mood changed regarding what women could and should do.

Cultural Competence in Nursing

Cultural competence is defined as possessing the skills and knowledge necessary to appreciate, respect, and work with individuals from different cultures. It is a concept that requires self-awareness, awareness and understanding of cultural differences, and the ability to adapt to clinical skills and practices as needed (London et al. 2003). In the Orthodox Jewish community, there are many strict cultural guidelines that the women must adhere to. Within the following paper I will provide examples that demonstrate why cultural competency is important in nursing.When seeking treatment in the Orthodox Jewish law,it permits men and women from being alone together unless they are close family member, or married to each other. This law applies when the women is being examined by a physician or a health care provider. For the Orthodox Jewish woman, a female provider is preferable, but the woman will choose the provider she feels is qualified to provide her with the best quality of care and who has the best reputation in his/her field (Abdelhak 2005).Spousal involvement in the delivery of a child is limited; a nurse may misunderstand a husbands lack of support as being neglectful to his wife, the nurse is not being culturally sensitive to the Orthodox couple. The nurse must understand according to the Jewish laws, if a woman is unclean with mucous discharge, bloody show, or amniotic fluid, The husband must exit the room as he is not allowed stay in the room with his wife while she is being examined, unless she is fully covered and will not be exposed to him.To be considered clean again after childbirth or menstruation , the women must go to a ritual bath called the † Mikveh†. The Orthodox Jewish women must consult with their Rabbi for approval of procedures or treatments; amniocentesis or elective cesarean sections. In such cases Orthodox Jewish couples may call their rabbi to ask for guidance on the subject or to get a blessing from him that all will go we ll. This would not be done in medical emergencies, such as a cesarean section for fetal distress or for inductions for medically indicated reasons (Abdelhak 2005).In the Orthodox Jewish community they believe in â€Å"Be fruitful and multiply†. It is Gods will how many children she will have, in this case the woman will avoid ever having a cesarean section as it can limit the amount of children she can have and she will not be able to fulfill Gods will. After childbirth, the nurse must be aware of the religious practices of naming a child. The woman will not fill her paperwork at the hospital, but rather fill it after the ceremony and return its afterwards.The giving of the name is thought to be a religious event and will lose significance if it is announced before either of these times (Abdelhak 2005). Orthodox Jews observe the Sabbath or Shobbas, which begins at sundown Friday evening and ends on Saturday evening. At this time no electrical appliance may be used or or any t raveling by car. If the orthodox Jewish woman is discharged the day of Shobbas; the nurse should know that she will not be able to leave the hospital until Shobbas has ended.To accommodate to her needs the nurse should make sure the woman has a meal before her discharge planning. in the Orthodox Jewish law it permits men and women from being alone together unless they are close family member, or married to each other. This law applies when the women is being examined by a physician or a health care provider. For the Orthodox Jewish woman, a female provider is preferable, but the woman will choose the provider she feels is qualified to provide her with the best quality of care and who has the best reputation in his/her field (Abdelhak 2005).Spousal involvement in the delivery of a child is limited. A nurse may feel that the husband is showing no spousal support or compassion to his wife. During the delivery the nurse can encourage him to give his wife support verbally, but the nurse must understand according to the Jewish laws, if a woman is unclean with mucous discharge, bloody show, or amniotic fluid. The husband may exit the room as he is not allowed stay in the room with his wife while she is being examined, unless she is fully covered and will not be exposed to him.To be considered clean again after childbirth or menstruation , the women must go to a ritual bath called the † Mikveh†. The Orthodox Jewish women must consult with their Rabbi for approval of procedures or treatments; amniocentesis or elective cesarean sections. In such cases Orthodox Jewish couples may call their rabbi to ask for guidance on the subject or to get a blessing from him that all will go well. This would not be done in medical emergencies, such as a cesarean section for fetal distress or for inductions for medically indicated reasons (Abdelhak 2005).In the Orthodox Jewish community they believe in â€Å"Be fruitful and multiply†. It is Gods will how many children she will have, in this case the woman will avoid ever having a cesarean section as it can limit the amount of children she can have and she will not be able to fulfill Gods will. After childbirth, the nurse must be aware of the religious practices of naming a child. The woman will not fill her paperwork at the hospital, but rather fill it after the ceremony and return its afterwards.The giving of the name is thought to be a religious event and will lose significance if it is announced before either of these times (Abdelhak 2005). Orthodox Jews observe the Sabbath or Shobbas, which begins at sundown Friday evening and ends on Saturday evening. At this time no electrical appliance may be used or or any traveling by car. If the orthodox Jewish woman is discharged the day of Shobbas; the nurse should know that she will not be able to leave the hospital until Shobbas has ended. To accommodate to her needs the nurse should make sure the woman has a meal before her discharge planning.

Communicate in a business environment Essay

3.1 Describe ways of verbally presenting information and ideas clearly 3.2 Explain ways of making contributions to discussion that help to move them forward 3.3 Describe methods of active listening 3.4 Explain the purpose of summarising verbal communications 4.1 Describe ways of getting feedback on communications 4.2 Explain the purpose of using feedback to develop communication skills Describe ways of verbally presenting information and ideas clearly to present any information or ideas I need clearly I often use simple language and short sentences this makes it easier for everybody to understand. Also I present the information is a variety of ways as some people understand things and concepts in different ways. For example; some people understand by hearing or seeing. Before I present any information or ideas I always plan out what I want to say, I often also take out any information that is not necessary. I also use active and personal language like ‘’you’ and ‘we’’. Explain ways of making contributions to discussion that help to move them forward To make conversation move forward, I often learn to listen to people and give importance for everybody’s ideas. This way I can make positive contributions that can lead to further discussion. I also often do not make a contribution to a subject who isn’t positive or may not affect me or my work. Describe methods of active listening In order to perform within LSG and to develop my skills listening is one the most important skills I should obtain. As it will portray the quality of my relationship with my team and clients. Listening is important as I need to often obtain information from others to learn new things. Methods of active listening include: Listening calmly without interrupting, so that I let the other person speak and show them that I care and respect them Asking others to repeat if I do not understand anything, in order to avoid mistakes Taking notes of important points, so that I do not forget or miss out on any important points Confirming what I have understood, so that there is no misunderstanding of information Explain the purpose of summarising verbal communications The purpose of summarising verbal communication is to identify major points, behaviours, thoughts and feelings that have been discussed. I then often collate all the information I have collected. By doing this is helps to have a clear precise outline of all communications. Describe ways of getting feedback on communications I believe that feedback completes the entire process of communication. Feedback helps us to decide if the communication was effects and useful. I often get feedback from my line manager Scott or I often get it from clients on the phone. If any feedback given is to improve on anything I often make note of the feedback and make a working progress for myself to include the suggestion in my work. Explain the purpose of using feedback to develop communication skills I use feedback for improve my work performance. It helps improve my work ethnic, team work and quality of my work. To help develop communication skills the feedback has to be received and acted upon. Once I have acted upon feedback I always let my line manager Scott know so that he can see I am willing to learn and enthusiastic and this may encourage people to offer me feedback in the future.

Womens role in the development of American consumer culture Essay

Womens role in the development of American consumer culture - Essay Example The business activities of the American women in the colonial period were oriented around the well-being of their home and family, so things like fashion were of tertiary importance to them. Unlike them, the modern American women get in fashion as soon as they reach adolescence. Modern American women smoke and drink in public, and embrace consumer culture. One way in which the role of women has significantly changed in the consumer culture in America since the colonial period is that women today have become the means of propagation of consumer culture both through consumption and through advertising. The ad of a new car is incomplete without a lady oozing sex appeal standing next to it in the poster. Women have become more of sexualized objects in the media unlike women of the colonial period. The sexual objectification is voluntarily portrayed in ads to draw increased attention of the consumers. Besides, gender equality and women empowerment have made women equally strong consumers today as

Purpose and Function of Newspeak Research Paper

Purpose and Function of Newspeak - Research Paper Example This shows that the language is widely used in Europe, particularly in Germany. The contextual theme of this study is based on a focus to clarify the purpose and principles of the Newspeak. As such, the study hopes to point out that in the event that the natural human atmosphere is affected by bad politics, the result is a corruption of the language; hence, the thoughts of people. Thus, this argument presents the main concept in support of inventing Newspeak. Largely, it can be argued that the invention decision of the language was informed by a desire to fulfill the ideological needs of the English Socialism or Ingsoc that was present by then. However, this form of language has been dormant and rarely used till later years such as 1984 since no one used Newspeak as the exclusive communication means, orally or written. During these periods, leading articles such as published by Times were written in the Newspeak language but required a the services of a specialist for the tour de force to take place. The expectation was that Newspeak would act as a replacement and supersede the Oldspeak (standard English) by around 2050 (Adams 60-72) However, the language steadily gained ground as all members in political parties at this time were optimistic and eager to incorporate the Newspeak words in their speeches. This was so that they would be recognized and defined by the new grammatical constructions that accompanied their language (Moustaki 50-6 1). At around the year 1984 when the uptake of Newspeak language was at its peak, the availed dictionary for the language through the Ninth and Tenth editions were provisional; hence, largely contained archaic and superfluous words and grammatical formations that required suppressions.